How much ampicillin?

martin LEACH leach at bu.edu
Fri Oct 4 10:07:13 EST 1996


I like 100.....cuts down on some satellites i used to get....50 works fine
though...others prefer this in the lab

M

Bill Burnett (bbur at wpo.nerc.ac.uk) wrote:

: Promega recommend a final concentration of ampicillin in 
: LB/ampicillin/IPTG/X-gal plates (for E. coli JM 109 transformed with 
: T-vector) of 100 ug/ml...   Can I get away with only 50 ug/ml as 
: suggested by a lab manual or is this a false economy?

: Anyone got a sure fire cheap plate recipe?

: Thanks!!

: B.

: -------------------------------------------------------------------------
: Bill Burnett: bbur at dml.ac.uk            
: Scottish Association for Marine Science
: P.O. Box 3, Oban, Argyll, PA34 4AD.



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