I like 100.....cuts down on some satellites i used to get....50 works fine
though...others prefer this in the lab
M
Bill Burnett (bbur at wpo.nerc.ac.uk) wrote:
: Promega recommend a final concentration of ampicillin in
: LB/ampicillin/IPTG/X-gal plates (for E. coli JM 109 transformed with
: T-vector) of 100 ug/ml... Can I get away with only 50 ug/ml as
: suggested by a lab manual or is this a false economy?
: Anyone got a sure fire cheap plate recipe?
: Thanks!!
: B.
: -------------------------------------------------------------------------
: Bill Burnett: bbur at dml.ac.uk: Scottish Association for Marine Science
: P.O. Box 3, Oban, Argyll, PA34 4AD.
--
..... Martin Leach Email:leach at bu.edu
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