Best oligo purification?

Steven Goldberg goldberg at bms.com
Fri Oct 4 07:56:28 EST 1996


In article <199610031715.MAA10699 at wustl.edu>, brett at BORCIM.WUSTL.EDU
(brett) wrote:

> >I would like to know what people think is the best, easiest and/or
> >fastest way to purify oligonucleotides for use as probes, PCR primers,
> >etc.  Thanks.
> >
I have been using NAP-10 columns from Pharmacia followed by EtOH
precipitation (using 1 mM MgCl2 and 2 vol EtOH) to clean up my oligos.  It
takes about 10 minutes after applying your crude sample to the column to
have clean oligo.  It performs very well for most appications.

Steve



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