E. coli strain for in vitro mutagenesis needed (dut-, ung-, F' )

[Vaclav Mach]

Hi there.

Will I find a kind person willing to send a dut-, ung-, F' E. coli
strain, such as CJ236 or BW313? This is needed for in vitro mutagenesis
and although this method is somehow out-dated, I would like to use it
since it is relatively cheep and avoids PCR.

Please contact me by E-mail: mach at entu.cas.cz. Thanks a lot.

             Sincerely,  Vaclav Mach