Making 2% skimmed milk in PBS

[martin LEACH]

Alex Chang (lpss at unixg.ubc.ca) wrote:
: I am trying to make 2% skimmed milk in PBS for blocking during my panning 
: procedures. I am wondering how to make it sterile. I know you can 
: autoclave it. I did it last time, the bottle returned to me with brown 
: solutions, I guess it was overdone. Well, our autoclaving is done by a 
: centralized service. That means we don't have control over the time and 
: temperture. I also tried filtration using 0.2 um filters, but the milk 
: solution clogged the filter easily.

: Any solutions to this problem?

did you try using several pre-filters in front of the 0.2 micron filter...

this works well for us when filtering chick embryo extract etc... and other
assorted goos....

later

Martin


: --
: Alex Chang
: Pathology
: University of British Columbia
: achang at hivnet.ubc.ca

--
.....          Martin Leach                Email:leach at bu.edu
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