IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Making 2% skimmed milk in PBS

martin LEACH leach at bu.edu
Thu Oct 3 16:55:53 EST 1996

Alex Chang (lpss at unixg.ubc.ca) wrote:
: I am trying to make 2% skimmed milk in PBS for blocking during my panning 
: procedures. I am wondering how to make it sterile. I know you can 
: autoclave it. I did it last time, the bottle returned to me with brown 
: solutions, I guess it was overdone. Well, our autoclaving is done by a 
: centralized service. That means we don't have control over the time and 
: temperture. I also tried filtration using 0.2 um filters, but the milk 
: solution clogged the filter easily.

: Any solutions to this problem?

did you try using several pre-filters in front of the 0.2 micron filter...

this works well for us when filtering chick embryo extract etc... and other
assorted goos....



: --
: Alex Chang
: Pathology
: University of British Columbia
: achang at hivnet.ubc.ca

.....          Martin Leach                Email:leach at bu.edu
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants
               USA                                on your

Webmaster of the biotech Rumor Mill

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net