Alex Chang (lpss at unixg.ubc.ca) wrote:
: I am trying to make 2% skimmed milk in PBS for blocking during my panning
: procedures. I am wondering how to make it sterile. I know you can
: autoclave it. I did it last time, the bottle returned to me with brown
: solutions, I guess it was overdone. Well, our autoclaving is done by a
: centralized service. That means we don't have control over the time and
: temperture. I also tried filtration using 0.2 um filters, but the milk
: solution clogged the filter easily.
: Any solutions to this problem?
did you try using several pre-filters in front of the 0.2 micron filter...
this works well for us when filtering chick embryo extract etc... and other
: Alex Chang
: University of British Columbia
:achang at hivnet.ubc.ca
..... Martin Leach Email:leach at bu.edu
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