I have two proteins, in two different amp-based expression vectors for
E. coli. Other than transforming with the two and analysing for
coexpression directly, is there any trick in order to get the two
vectors into the bacteria?
On expression one protein is pretty toxic to the cells, so is it likely
there would be selection for those cells without this vector.
Is the best option to clone one of the proteins into an alternative
expression vector based on a different antibiotic e.g. kanamycin and
select on amp-kan media? Are there any particular combinations of
antibiotic that it is not advisable to use? Would it be going too far
to use say BL21 (DE3) pLys S and select on amp-kan-chloramphenicol?
I'd be grateful for any advice.
crbaker at mail.tcd.ie