Co-expression of two proteins in E.coli

C.R.Baker crbaker at mail.tcd.ie
Fri Oct 4 02:21:33 EST 1996


I have two proteins, in two different amp-based expression vectors for 
E. coli. Other than transforming with the two and analysing for
coexpression directly, is there any trick in order to get the two 
vectors into the bacteria?
On expression one protein is pretty toxic to the cells, so is it likely
there would be selection for those cells without this vector.
Is the best option to clone one of the proteins into an alternative
expression vector based on a different antibiotic e.g. kanamycin and 
select on amp-kan media? Are there any particular combinations of 
antibiotic that it is not advisable to use? Would it be going too far
to use say BL21 (DE3) pLys S and select on amp-kan-chloramphenicol?
I'd be grateful for any advice.

Cara Baker
crbaker at mail.tcd.ie



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