ligation of PCR products

M. Aitchison m.j.aitchison at ncl.ac.uk
Fri Oct 4 10:18:54 EST 1996


Hi there,

just to make sure: am I right in assuming that PCR products have no 5' 
phosphate groups, and hence need kinase treatment before they can be ligated 
to each other?

can both be done in the same reaction (tube)?

thanks a lot!

alois.




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