ligation of PCR products

Francois Coulier coulier at infobiogen.fr
Sat Oct 5 01:49:43 EST 1996


On 4 Oct 1996, M. Aitchison wrote:

> Hi there,
> 
> just to make sure: am I right in assuming that PCR products have no 5' 
> phosphate groups, and hence need kinase treatment before they can be ligated 
> to each other?
> 
> can both be done in the same reaction (tube)?
> 
> thanks a lot!
> 
> alois.
> 
> 
> 
PCR product have the 5' of the primers. So, first kinase your primers.

Francois Coulier
INSERM Unite 119
27 bd Lei Roure
13009 Marseille
France

tel (33) 91 75 84 11 (October 18, 1996=> 33 (0) 4 91 75 84 11)
fax (33) 91 26 03 64 (October 18, 1996=> 33 (0) 4 91 26 03 64)
email: coulier at infobiogen.fr




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