In article <534dfb$rsq at babyblue.cs.yale.edu> gary vanzin,
gfv94001 at uconnvm.uconn.edu writes:
>>I'm looking for a reference/protocol to purify hybrid DNA using a
>biotin/streptavidin system. I'm screening 6900 arabidopsis plants,
>each containing 1-4 insertional mutations (T-DNA). I would like to
>enrich my DNA preps for the T-DNA, so I can combine 100,500, or maybe
>even all 6900 DNA preps during the subsequent PCR screen. I believe I
>can do this using biotinylated T-DNA as a probe, then doing
>hybrid-select, and purification over a streptavidin column. Any Ideas?
> Anybody know how well streptavidin coated dynalbeads work?
I used the promega streptavidin beads for selection of biotin probe/cDNA
clones.
As long as there is a PCR step, the yield is not really a big problem.
You can label RNA or DNA with biotin by enzymatic incorporation,
photobiotin, psorelen biotin, or nick translation or random priming with
biotin nuc's.
**my opinions are just that**
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Eric Lader Ph.D. Senior R&D Scientist,
Ambion Inc.
Unique tools for Molecular Biology Research
Phone (800) 888-8804 Fax (512) 445-7139
(512) 445-6979 lader at ambion.com
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