I have one question regarding the Chomczynski-Sacchi method for RNA prep
with GIT: Do you need to modify the protocoll for bacteria? We have had
problems with those, although it works out OK in mammalian cells. Is
there a modified protoll available? In Current Protocolls, we only found
a modification of the Cesium Cloride protocol.
A reference would be great.
Phone: int-46-31-602588; or 602445 (lab), or 603461 (secretary). Fax
E-mail joj at ss.gu.se. Mailing specification: Gröna Stråket 8,
Sahlgrenska University Hospital,
S-413 45 Göteborg, Sweden.