I'm looking for a reference/protocol to purify hybrid DNA using a
biotin/streptavidin system. I'm screening 6900 arabidopsis plants,
each containing 1-4 insertional mutations (T-DNA). I would like to
enrich my DNA preps for the T-DNA, so I can combine 100,500, or maybe
even all 6900 DNA preps during the subsequent PCR screen. I believe I
can do this using biotinylated T-DNA as a probe, then doing
hybrid-select, and purification over a streptavidin column. Any Ideas?
Anybody know how well streptavidin coated dynalbeads work?
gfv94001 at uconnvm.uconn.edu