I've been having trouble reading my sequencing gels because the
bands tend to get blurry after about 60-100 bases from the bottom
and I can't tell if I'm looking at single bands or doublets. I've
been running 5% acrylamide gels at 35-40 watts. The buffer is
1xTBE, except that I add sodium acetate to 1M in the bottom buffer
after the gel has run about half way. Any suggestions on sharpening
my bands would be grealy appreciated.