Hep G2 transfection with lipofectin

[Michele Dowless]

Hi everyone,

Does anyone have any experience transfecting HepG2 cells using Life 
Technology's Lipofectin reagent?  I have tried a couple of times but can't 
seem to get the conditions right and the cells end up dying after 3 days.  I 
am especially interested in cell density, and amount of plasmid and lipid 
used.  Any references would also be appreciated.

Please email me as well as post to newsgroup as I don't want to miss any 
replies.

Thanks in advance,
Michele

mdowless at lilly.com