Does anyone have any experience transfecting HepG2 cells using Life
Technology's Lipofectin reagent? I have tried a couple of times but can't
seem to get the conditions right and the cells end up dying after 3 days. I
am especially interested in cell density, and amount of plasmid and lipid
used. Any references would also be appreciated.
Please email me as well as post to newsgroup as I don't want to miss any
Thanks in advance,
mdowless at lilly.com