HELP! Protein purification in presence of gelatin/BSA

Dima Klenchin klenchin at facstaff.wisc.edu
Fri Oct 4 13:36:56 EST 1996


In article <3255447B.10BA at wesleyan.edu>,
   tguina at WESLEYAN.EDU (tina guina) wrote:
->Biologically, this protein is a cytolysin which inserts into the target 
->membranes and form pores.

->1) what would be the best method to concentarte the protein?
->	  (is ammonium sulfate good enough and would it precipitate the 
gelatin 		    
->    as well)

Depends on at what % AS it is ppted. Just try it.

One thought: if it inserts itself into membranes, then
how about mixing your medium with lyposomes and then
pelleting them down. Sounds like it might be extremely 
effecient purification/concentration step.

Good luck.

- Dima



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