Luciferase half-life?

[Ferland Louis H.]

Martin, John, and all the rest of you, Netters,

The half-life of the protein is only half of the story. I am in a 
position similar to John's i.e. I want to verify possible (expected) 
rapid inhibition of a transfected promoter driving the LUC reporter. But, 
to have an idea of whether LUC is useable for this type of experiment, we 
need not only the half-life of the LUC protein, but also of its mRNA. 
Does anyone have any data on this?

We're just going to give it a try...

- Louis


On 2 Oct 1996, martin LEACH wrote:

> Date: 2 Oct 1996 20:19:26 GMT
> From: martin LEACH <leach at bu.edu>
> To: methods at net.bio.net
> Subject: Re: Luciferase half-life?
> 
> Hi john
> 
> I believe the half life of luciferase is around 4.5 hours....(protein)
> I believe this was determined in NIH fibroblasts...not sure though
> 
> Martin
> 
> jstrassw at OPAL.TUFTS.EDU wrote:
> : Hello!
> : I would like to use a fos-luciferase reporter in HeLa cells.  I will 
> : co-transfect a plasmid to express what I hope is an inhibitor of 
> : signalling and will be reflectedin a decrease in luciferase activity.
> 
> : The question: what is the half-life of luciferase in hela cells?  When I 
> : starve then stimulate the cells with serum, then azzay, I will have 
> : serium-induced luciferase as well as background luciferase.
> 
> : Any ideas?
> : Thanks,
> : John
> 
> 
> : ------------------------------------------------------------------------
> : John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
> : Tufts - New England Medical Center     | Fax   (617) 636 6190
> : Box 166                                | Email:  jstrassw at opal.tufts.edu
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> 
> --
> .....          Martin Leach                Email:leach at bu.edu
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Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757     FAX: (514) 843-2719