IMPACT kit from new england biolabs...

Arioch siyer at bmg.bhs.uab.edu
Sat Oct 5 17:56:49 EST 1996


hey all,
        just saw this in the september 6th issue of cell.  this is a new
kit from NEB that claims that you can get pure NATIVE protein (no fusions)
in "one" step.  basically they use proteins called inteins that are fused
to YPOI.  this undergoes directed cleavage when you add DTT (cleave a S-S
bond somewhere right before the fused region) and you get native protein
with no fusion domain to it.  anybody try this and if so, what is the
yield like??  how good is this system?  seems like a cool idea, but as
alwayz with any kit, i'm sceptical...any info on this would be
apprciated...thanx much...

-- 
Arioch, Lord of Chaos
siyer at bmg.bhs.uab.edu
graduate student; dept of biochemistry and molecular genetics
university of alabama at birmingham



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