de-salting prior to SDS-PAGE loading...

Arioch siyer at bmg.bhs.uab.edu
Sat Oct 5 17:45:40 EST 1996


hey all,
           anybody know of a good protocol to remove the salt from samples
before they are loaded on to SDS-PAGEs??  i have found out that hi salt
tends to distort the way the bands run on the gel (screwing up the
electrical field and therefore affecting protein mobility).  i found this
one protocol that tells you to add 0.15% deoxycholic acid, incubate for 10
min followed by addition of TCA ---> spin for about 15 min at 3000g --->
decant all the TCA and resuspend pellet (containing precipitated protein
with presumably no salt).  this however has not been too successful (for
me) and still causes distortion of my gel bands (much annoyance).  anybody
know of any modicfications to this protocol or other protocols that
work??  thanks much...

-- 
Arioch, Lord of Chaos
siyer at bmg.bhs.uab.edu
graduate student; dept of biochemistry and molecular genetics
university of alabama at birmingham



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