help!!! for plasmid YIElD

Takbum Ohn ahnss at hitel.kol.co.kr
Sun Oct 6 09:01:06 EST 1996


Hello!! netters...

I have cloned a cell wall hydrolase (probably toxic to E. coli host) from
Moraxella sp. and now, I subcloned the insert as short as 1 kb. 

The subclone expressed the gene very highly  but, oddly, the copy of the
subclone plasmid became very low. 

I don't know what make my plasmid like that.

The mother plasmid is pT7T3 19u, a high-copy number phasimid vector.

Any help would very appreciated.

Thanks..

Tak-Bum Ohn
Department of Biology
Hanyang University
KOREA

Personal E. mail address: ahnss at hitel.kol.co.kr
 



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