I have cloned a cell wall hydrolase (probably toxic to E. coli host) from
Moraxella sp. and now, I subcloned the insert as short as 1 kb.
The subclone expressed the gene very highly but, oddly, the copy of the
subclone plasmid became very low.
I don't know what make my plasmid like that.
The mother plasmid is pT7T3 19u, a high-copy number phasimid vector.
Any help would very appreciated.
Department of Biology
Personal E. mail address: ahnss at hitel.kol.co.kr