sequence is blurry
jwhitewa at chat.carleton.ca
Sun Oct 6 13:58:42 EST 1996
gc (genecutl at mendel.berkeley.edu) wrote:
> I've been having trouble reading my sequencing gels because the
> bands tend to get blurry after about 60-100 bases from the bottom
> and I can't tell if I'm looking at single bands or doublets. I've
> been running 5% acrylamide gels at 35-40 watts. The buffer is
> 1xTBE, except that I add sodium acetate to 1M in the bottom buffer
> after the gel has run about half way. Any suggestions on sharpening
> my bands would be grealy appreciated.
We generally use an 8% gel run at 60 Watts and get clear bands.
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