Lou Cantolupo wrote:
>> Does anyone have a good protocol for isolating low copy # pDNA from
> bacteria?I have a method out of Maniatis -
- grow 30ml of culture in 2YT with antibiotic, until late log phase
(OD600 = 0.6)
- innoculuate 500ml 2YT broth with 25ml of above, use a 2 litre flask.
Incubate for 2.5 hours at 37oC, shaking 300 cycles per min (OD 600 should
be about 0.4)
- add 2.5ml of chloramphenicol (34mg/ml in EtOH), incubate for further
12-16 hours, 37oC, shaking
- treat as normal maxiprep
Hope this is of some help
Lynn
