Nylon-vs-nitrocellulose??

[dna]

In article <32558BD8.3928 at nbnet.nb.ca>, soniag at nbnet.nb.ca says...
>
>Hi,
>
>I'm wondering what you, the "experts" prefer for southern blots:  nylon
>or nitrocellulose???....and what should I use.
>
>Thanks,
>

Hi,

Nylon by a long shot.
We went though this a couple of years ago and tested all sorts of 
different membranes from different companies.  What we found was that for 
DNA southerns with non rad. probes, MSI magnagraph won hands down.
This can be bought through Fisher.  MSI Nitropure (nylon supported nitro) 
was the best for protein westerns.   


-- 
John R. McQuiston
Molecular Genetics Lab
CMMID VMRCVM
VPI & SU
1410 Prices Fork Rd
Blacksburg VA 24061