Hi
Yes, we use the 9600 machine for sequencing. It works just fine. The
reaction is done under a twenty microliter reaction volume according to
the ABI PRISM kit instructions. I never noticed significant loss.
Hope this helps
Olivier
Jan Hoolwerf wrote:
>> Hello,
>> At this moment we are using a Perkin Elmer 480 for most of our work.
>> One of the applications is sequencing with the Amersham ThermoSequenase
> fluorescent labelled primer cycle sequence kit RPN 2436 and with this kit we
> use a volume of 12 microliters covered with a drop of oil to prevent
> evaporation.
>> No problems with the reaction but the use of oil (also in the PE 480) is a
> messy thing and especially with those small volumes it's something i really
> would like to give up.
>> So, since we also have a PE 9600 cycler the obvious thought is to do all the
> work in that machine. No oil, in or outside the tubes, small tubes... it seems
> like heaven. <g>
>> But my concern is the small volume i'm using. Simply stated i'm afraid i will
> loose to much of the 12 microliters.
>> Has anyone experience with the PE 9600 for this type of work? Any tips to
> share? An optimal program perhaps?
>> with regards, Jan Hoolwerf
>> ******************* these words were mine, all mine !! ********************
> J.D. Hoolwerf
> Neth. Inst. for Dairy Research
> P.O. Box 20 phone : 31-318-659511
> 6710 BA Ede fax : 31-318-650400
> the Netherlands e-mail : hoolwerf at solair1.inter.nl.net> ***************************************************************************
