PE 9600 for cycle sequencing ?

Olivier Gandrillon ogandril at cri.ens-lyon.fr
Mon Oct 7 07:26:28 EST 1996


Hi

Yes, we use the 9600 machine for sequencing. It works just fine. The 
reaction is done under a twenty microliter reaction volume according to 
the ABI PRISM kit instructions. I never noticed significant loss.

Hope this helps

Olivier

Jan Hoolwerf wrote:
> 
> Hello,
> 
> At this moment we are using a Perkin Elmer 480 for most of our work.
> 
> One of the applications is sequencing with the Amersham ThermoSequenase
> fluorescent labelled primer cycle sequence kit RPN 2436 and with this kit we
> use a volume of 12 microliters covered with a drop of oil to prevent
> evaporation.
> 
> No problems with the reaction but the use of oil (also in the PE 480) is a
> messy thing and especially with those small volumes it's something i really
> would like to give up.
> 
> So, since we also have a PE 9600 cycler the obvious thought is to do all the
> work in that machine. No oil, in or outside the tubes, small tubes... it seems
> like heaven. <g>
> 
> But my concern is the small volume i'm using. Simply stated i'm afraid i will
> loose to much of the 12 microliters.
> 
> Has anyone experience with the PE 9600 for this type of work? Any tips to
> share? An optimal program perhaps?
> 
> with regards, Jan Hoolwerf
> 
> ******************* these words were mine, all mine !! ********************
>  J.D. Hoolwerf
>  Neth. Inst. for Dairy Research
>  P.O. Box 20                        phone  : 31-318-659511
>  6710 BA  Ede                       fax    : 31-318-650400
>  the Netherlands                    e-mail : hoolwerf at solair1.inter.nl.net
> ***************************************************************************



More information about the Methods mailing list