In article <siyer-0510961822540001 at bmg146.cmc.uab.edu>, Arioch
<siyer at bmg.bhs.uab.edu> writes
>hey all,
> just saw this in the september 6th issue of cell. this is a new
>kit from NEB that claims that you can get pure NATIVE protein (no fusions)
>in "one" step. basically they use proteins called inteins that are fused
>to YPOI. this undergoes directed cleavage when you add DTT (cleave a S-S
>bond somewhere right before the fused region) and you get native protein
>with no fusion domain to it. anybody try this and if so, what is the
>yield like?? how good is this system? seems like a cool idea, but as
>alwayz with any kit, i'm sceptical...any info on this would be
>apprciated...thanx much...
They are using a modified intein and from data I saw presented at a
conference a few weeks back it is a very promising system. My only
queries are:
1. Is if the affinity column is reusable?
2. How expensive is the affinity column 'cos it's OK purifying
proteins on a shake flask scale but I need to work on a much larger
scale.
Duncan
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My mind's made up. Don't confuse me with the facts!
Duncan Clark
DNAmp Ltd.
http://www.dnamp.com
