In article <32519D66.7BD7 at ss.gu.se>, John-Olov Jansson <joj at ss.gu.se> wrote:
>> I have one question regarding the Chomczynski-Sacchi method for RNA prep
> with GIT: Do you need to modify the protocoll for bacteria?
It is the experience of several people in our lab that to get reliable RNA
from E. coli or Salmonella you need to use a hot phenol protocol. It
seems that the average mRNA half-life is just to short or the commercial
methods don't recover all of the transcripts quantitatively. I personally
didn't have luck with the C-S protocol and another person in the lab
didn't have satisfactory results with C-S or the Qiagen RNA isolation
kit. It's a pain, but I can isolate 1.2-1.5 mg of RNA that is in good
If you are interested let me know and I'll post a copy.
Best of luck,
Brad Nicholson |"If it worked the first time,
Department of Pathology | it wouldn't be research.'
University of Utah | ...Brad Nicholson
Salt Lake City, UT 84132 | My opinions are solely my own.
brad_nicholson at hlthsci.med.utah.edu |
or: (801)-581-4365 | iligitimi non corborundrum