Re-PCRing after gel-purification problems

Brendan barry at sccs.swarthmore.edu
Mon Oct 7 10:13:52 EST 1996


Hi,

   I'm having trouble simply Re-PCRing products after gel purifying them. 
I use TAE gels and SpinX columns to gel purify first round bands.  Why is
this second round not working?  Does PCR work with templates that are in
Acetate (tAe)?  Any ideas....I'm stumped.

Barry Mook
 
barry at sccs.swarthmore.edu



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