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DIG vs 35S for in situ hybridization

William Sun william at neuro.usc.edu
Mon Oct 7 16:12:04 EST 1996

Hello all,

I wish to do some in situ hybridization studies looking at the expression
of a specific gene in rat and rabbit brain sections.  I would like to
know the advantages and disadvantages of using DIG and 35-S as the means
for detection.  Also, I am considering using riboprobes.  If you have
reasons as to why DNA oligos might work better, please let me know. 
Thanks for any advice/suggestions.


William Sun, Ph.D.                     Phone: (213)740-3406
Neuroscience Program                   FAX:   (213)740-5687
University of Southern California      Pager: (310)243-9878 
Los Angeles, CA 90089-2520             http://www-bcf.usc.edu/~wisun/

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