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Betaine in PCR

Debbie Moore biof020 at uabdpo.dpo.uab.edu
Mon Oct 7 10:51:40 EST 1996

Does anyone have any experience in using betaine in PCR?  How much do you
need to lower the annealing temp?  The reference I have suggests a 2M
final concentration.  Any info would be appreciated.  Thanks
Debbie Moore
DMoore at uab.edu

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