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DNA from paraffin tissue section.

Gregory Jacobson gmj at waikato.ac.nz
Mon Oct 7 15:55:05 EST 1996

I need get some DNA from part of a tissue section (Breast Ductal
Carcinoma) for PCR and subsequent CFLP or SSCP.  I have removed the
paraffin from the sections using xylene and alcohol washes. I will use a
silcon pen to isolate the tumour cells.  

 I would be grateful for advice about a method for protease treating the
tissue :
  Is TE the best buffer to use?
  How do I apply the proteinase K? (With buffer? or after soaking tissue in TE?)
  Should I incubate the slide at 42 degrees C? (higher?)  
  Can I simply pipette the DNA off the tissue section after proteinase K  

 Thanking you in advance,
  Gregory Jacobson 
  2nd year MSc
  Uni. of Waikato
  New Zealand

      Please email : gmj at waikato.ac.nz

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