I need get some DNA from part of a tissue section (Breast Ductal
Carcinoma) for PCR and subsequent CFLP or SSCP. I have removed the
paraffin from the sections using xylene and alcohol washes. I will use a
silcon pen to isolate the tumour cells.
I would be grateful for advice about a method for protease treating the
Is TE the best buffer to use?
How do I apply the proteinase K? (With buffer? or after soaking tissue in TE?)
Should I incubate the slide at 42 degrees C? (higher?)
Can I simply pipette the DNA off the tissue section after proteinase K
Thanking you in advance,
2nd year MSc
Uni. of Waikato
Please email : gmj at waikato.ac.nz