DIG labelling and detection

[Harold Rees]

Are you using colorimetric detection or chemiluminescence?  The 
chemiluminescent detection is much more sensitive than colorimetric.  The 
latter technique is actually not recommended for single-copy genes 
because of its lack of sensitivity.  We have been using CSPD from 
Boehringer for our reactions, and it works well.  For maximum 
sensitivity, however, radioactive is still the best way to go.

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Hal Rees                                ********************************
Monell Chemical Senses Center           *"Oh well, whatever, nevermind"*
Philadelphia, PA                        *  -Kurt Cobain                * 
hrees at mail.sas.upenn.edu                ********************************