Help destaining NBT/BCIP Western blot on Nitrocellulose

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Mon Oct 7 10:21:16 EST 1996


Dear Bionetters,

Does anyone know of a method to destain (or reduce the amount of
over-staining) of a western blot that was detected with alkaline-
phosphatase secondary antibody and stained with NBT/BCIP on
nitrocellulose?  The blot was overdeveloped in the dye reagent
and it is difficult to see the bands of interest.

Or is there a way to wash off the current stain and antibodies
leaving the blotted proteins on the NC thus allowing re-probing
of the original blot?

Thanks, 
Brad


**************************************************************
		    Bradley Turner
	         Beth Israel Hospital

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			turner at sprcore.bih.harvard.edu
330 Brookline Avenue		bsturner at mbcrr.harvard.edu
Boston, MA 02215		bturner at bih.harvard.edu
**************************************************************





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