Dear Bionetters,
Does anyone know of a method to destain (or reduce the amount of
over-staining) of a western blot that was detected with alkaline-
phosphatase secondary antibody and stained with NBT/BCIP on
nitrocellulose? The blot was overdeveloped in the dye reagent
and it is difficult to see the bands of interest.
Or is there a way to wash off the current stain and antibodies
leaving the blotted proteins on the NC thus allowing re-probing
of the original blot?
Thanks,
Brad
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Bradley Turner
Beth Israel Hospital
Harvard Medical School 617-667-1215 phone
Division of Gastroenterology 617-667-2767 fax
Room Dana 536 turner at sprcore.bih.harvard.edu
330 Brookline Avenue bsturner at mbcrr.harvard.edu
Boston, MA 02215 bturner at bih.harvard.edu
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