Making 2% skimmed milk in PBS

Technical Assistance TechAssist at pierce.geis.com
Tue Oct 8 15:07:11 EST 1996


Robert Willows wrote:
> 
> We routinely use 15mM Sodium Azide in our 2% skim milk to stop bacterial
> growth. Although, I don't think you can do this if you use a HRP
> conjugated secondary because I think the azide is inhibitory.
> 
> --
> Robert Willows
> Email: Robert_Willows at brown.edu

Azide is indeed an inhitor of HRP activity. However, at such low concentrations, 
it usually is not a problem. An alternative would be to add thimerasol.



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