oligos vs others
Barney P. Crum
00bpcrum at bsu.edu
Tue Oct 8 13:58:04 EST 1996
I'm currently doing northerns using a 35mer oligo to probe for a specific
RNA sequence. I am having trouble getting the necessary signal generated
and was wondering if other probes may work better for Northern hybridization
(i.e. a larger probe or RNA probe). I know that the mRNA is in high enough
abundance to detect it using in situ hybridization. Any ideas will greatly
help. Thanks.
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