RNA isolation from brain tissue...help!
shelley at DARWIN.SFBR.ORG
Tue Oct 8 12:18:17 EST 1996
I have isolated total RNA from brain tissue using several micro-scale
kits that use guanidine thiocyanate, and, depending on the prep, I have
gotten 10 to 50 ug of RNA from 150mg of starting tissue. I have used
gross brain tissue, and in my hands, cerebellum, cortex, hippocampus,
hypothalamus have all given variable amounts of total RNA per 150mg of
I have also isolated poly(A+)-enriched RNA from choroid plexus and
hypothalamus using Invitrogen's micro-scale FastTrack kit, and gotten ng
quantities (enough to amplify a portion of my gene of interest using
Hope this helps some.
Southwest Foundation for Biomedical Research
On 8 Oct 1996, Chris Woodcock wrote:
> I am going to be isolating RNA from very small amounts of brain tissue.
> Does anyone out there have any methods for use with very small
> quantities? In addition does anyone have a feel for how much RNA yield
> varies between different brain regions and/or what the yields are likely
> to be?
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