I'm doing recombinant PCR to insert a fragment of 30 bps into a known
1kb sequence. Of course, in an effort to minimize costs, I assumed that 5
bp's overlap would be quite enough. What is the minimum number of bp's
that still works nicely? Has anyone tried with just 5 bp's over a total
final PCR product of nearly 1 kb? Thanx for any info....
barry at sccs.swarthmore.edu