How many bases overlap in recombinant PCR?

Brendan barry at sccs.swarthmore.edu
Tue Oct 8 09:18:58 EST 1996


Hi,

   I'm doing recombinant PCR to insert a fragment of 30 bps into a known
1kb sequence.  Of course, in an effort to minimize costs, I assumed that 5
bp's overlap would be quite enough.  What is the minimum number of bp's
that still works nicely?  Has anyone tried with just 5 bp's over a total
final PCR product of nearly 1 kb?  Thanx for any info....

Barry Mook
barry at sccs.swarthmore.edu



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