Re-PCRing after gel-purification problems

Gerd Nilsen gerdn at ibg.uit.no
Tue Oct 8 06:56:27 EST 1996


Hello
I have had no problems with re-PCR, but I haven¥t tried the same method
as you. I used low-melting point agarose gels, glass-milk, or
freeze-squeze metods to get the DNA out of the gel. Which method depents
on the size of the band. More than 500 bp, glassmilk is quicker. Less
than 500 bp; - glassmilk won¥t always let the DNA go, so I prefer
freeze-squeze (modified by diluting the agarose pieces in 0.3 M NaAc pH
7.0, before freezing).

Gerd





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