Nested Deletions Kits for Sequencing
Joao Vasconcelos Costa
jcosta at pen.gulbenkian.pt
Tue Oct 8 03:04:11 EST 1996
J. Pat Martinez wrote:
> I need to sequence a 10 kb fragment and will use a strategy based on a
> series of nested deletions. I'm aware of two different strategies for
> generating nested deletions. One strategy uses Exonuclease III and
> Mungbean nuclease, and the other uses transposon mediated deletions
> (Deletion factory by BRL). I was leaning toward the Exonuclease/Mungbean
> nuclease based kits. Any suggestions on which manufacture to go with,
> such as Promega's Erase a Base kit or Stratagene's deletion kit. Thanks
> for your suggestions.
> Pat M.
Nested deletions is one of my most frequent approaches to sequencing. I
have used successfully both kits you refer and I found no significant
differences between them.
The same is true for what I use more commonly: my own reagents, which
are much cheaper.
***** JOAO VASCONCELOS COSTA, MD, PhD
***** Instituto Gulbenkian de Ciencia - Oeiras, Portugal
***** mailto:jcosta at pen.gulbenkian.pt
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