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Large DNA cloning?

Yi Zhang (BIO) yzhang1 at chuma.cas.usf.edu
Tue Oct 8 21:37:57 EST 1996

Hi there:

I am going to clone a large peice of viral DNA fragment into pPAC vector.
This insert is 150 kb long. I have never done such a big piece of DNA
cloning before. I would like to get help from anyone who has done such or
who knows any literature (technical) on how to isolate this DNA from virus
without break the DNA, and what the best way is to transform the DNA into
E. coli host (chemical competent cells or electroporation) .

Thank you in advance.


						* ___
Yi (Ian) ZHANG,					*   | | /
IP2CMB, IBS					* --- |/
USF, Tampa, FL					* |__----
Web site: http://www.ualberta.ca/~yz1/index.html*   | |\
						*   | |/\
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