I'm trying to isolate mRNA from human peripheral monocytes, and am having
no luck. I've been using Pharmacia's Quick-Prep micro kit. Can anyone
who has experience isolating mRNA from phagocytic cells give me a hint
on how to make this work successfully? I'm getting zero yield.
Email is preferable, but any information is useful.
Jay Kugler University of Chicago, Department of Pharm./Phys.
kug1 at midway.uchicago.edu