mRNA isolation from monocytes

jay kugler kug1 at midway.uchicago.edu
Tue Oct 8 17:56:20 EST 1996


I'm trying to isolate mRNA from human peripheral monocytes, and am having
no luck.  I've been using Pharmacia's Quick-Prep micro kit.  Can anyone
who has experience isolating mRNA from phagocytic cells give me a hint
on how to make this work successfully?  I'm getting zero yield.

Email is preferable, but any information is useful.

-------
Jay Kugler             University of Chicago, Department of Pharm./Phys. 
kug1 at midway.uchicago.edu



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