Best oligo purification?
lwashing at sunflower.bio.indiana.edu
Tue Oct 8 17:04:22 EST 1996
In article <530kvi$e2f at maze.dpo.uab.edu>, levy at uab.edu (David N. Levy) wrote:
> I would like to know what people think is the best, easiest and/or
> fastest way to purify oligonucleotides for use as probes, PCR primers,
> etc. Thanks.
A Mono-Q (ion exchange) column works great, is fast and easy and cheap (if
you have an FPLC unit or equivalant LC system) but doesn't seem to resolve
oligos longer than about 30 bases long. Acrylamide gels give excellent
separation for any size oligo.
But (as has been noted) for most applications you can use them unpurified
if they were well made to begin with.
Indiana Molecular Biology Institute
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