Specificity of PCR primers.

Zhongtang Yu zyu at UNIXG.UBC.CA
Tue Oct 8 23:42:43 EST 1996


I am trying to design a PCR primer for a specific 16S rRNA.  I wonder if a 
primer (a 20mer) which has complete match with my target but only ONE 
mis-match with other related 16S rRNAs can be use to amplify only my target. 
My rRNA will be from mixed microbial community.  Any info will be 
appreciated.


Zhongtang Yu
Dept. of Microbiol. UBC.



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