I am trying to design a PCR primer for a specific 16S rRNA. I wonder if a
primer (a 20mer) which has complete match with my target but only ONE
mis-match with other related 16S rRNAs can be use to amplify only my target.
My rRNA will be from mixed microbial community. Any info will be
appreciated.
Zhongtang Yu
Dept. of Microbiol. UBC.
