I have a protein that I want to express which is toxic to E.coli. I
would like to get my hands on an E. coli system in which cells
are infected with a T7 polymerase-containing phage rather than adding
IPTG to express an endogenous T7 polymerase. If anyone knows where I
can find a system like this I would be most grateful.
...doom is like the handle of a pot, | stop waiting, it's
it's there, know it, have ice in your| already happened.
tea, marry, have children, visit your| genecutl at mendel.berkeley.edu
dentist, do not scream at night even |
if you feel like screaming... -cb |