T7 Phage expression system

gc genecutl at mendel.berkeley.edu
Tue Oct 8 22:51:51 EST 1996


I have a protein that I want to express which is toxic to E.coli.  I
would like to get my hands on an E. coli system in which cells
are infected with a T7 polymerase-containing phage rather than adding
IPTG to express an endogenous T7 polymerase.  If anyone knows where I
can find a system like this I would be most grateful.

--gc

...doom is like the handle of a pot, |        stop waiting, it's
it's there, know it, have ice in your|         already happened.
tea, marry, have children, visit your|   genecutl at mendel.berkeley.edu
dentist, do not scream at night even |
if you feel like screaming...   -cb  |



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