Sequencing 7-8 kb deletion

Sharon Jean Kelley skelle2 at unix1.sncc.lsu.edu
Wed Oct 9 11:55:51 EST 1996


 
Hi!
 
I wish to sequence a large deletion to determine its break points.  I
know from restriction analysis that a 13 kb region of lambda DNA has been
reduced to 5 kb.  Any advice on how to tackle this problem would be
appreciated.  I have experience with cycle sequencing using S35 labeled
dATP.  I do not have access to an automated sequencer.
 
Thanks in advance for any advice.
 
Sharon
 



More information about the Methods mailing list