In article <newitt-0910961330520001 at 18.104.22.168>,
newitt at nih.gov (John A. Newitt) wrote:
->In article <mac446-0410961737230001 at hastingsq610.harvard.edu>,
->mac446 at hastingslab.harvard.edu (Thomas F. Fagan) wrote:
->> Isn't it a matter of unfolding? The more concentrated the enzyme is
->> more likely it is to be bound to other enzyme molecules by hydrogen
->> bonding or other interactions and the less likely it is to
->> unfold. This is why you can substitute "enzyme" with BSA or some
->> stabilizing protein.
->I think sticking to surfaces has a lot to do with it. A large percentage
->of your protein will be lost due to nonspecific sticking to the tube,
->when it is too dilute.
I don't think so. Long time ago I compared stability of very dilute
IgG-peroxidase conjugates incubated in either control tubes and in
tubes extensively preblocked (and washed) with BSA. A loss of
activity due to sticking contributed minor portion of the overall loss.