If you are talking about the pZerO from Invitrogen, then yes, I have used
it and it does work. Please follow their protocol. Any "nibbling" done by
restriction enzymes on the vector will lead to background as well as long
term storage of isolated linear vector will also lead to higher background.
When done "by the book", background is really zero (well, of course, that
depends on how many colonies you feel like screening...).
Richard J. Daines
rdaines at q.continuum.net
Gerald Downes <gerry at MORPHEUS.WUSTL.EDU> wrote in article
<Pine.LNX.3.91.961009171722.8186A-100000 at morpheus.wustl.edu>...
>> I received a pamplet from Clontech that describes their
> pZErO cloning vector. Of course it *sounds* like heaven on earth (ease
> of use with no background) but is there a catch? Does anyone have
> experience with this vector or another that is supposed to give
> virtually no backgroud (CIP treated vectors excluded).
> Gerald B. Downes, Gautam Lab
> NIGMS Pre-doctoral Fellow
> Neuroscience Program
> Washington University Medical School
> Box 8054, St. Louis MO. 63110
> Ph. (314)362-8526
> Fax (314)362-8571