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IPTG concentration for induction?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Oct 9 11:08:33 EST 1996

In article <deusebe-0910961556130001 at cherubin.ens.fr>, =?iso-8859-
1?q?Dani=E8le_EUSEBE?= <deusebe at wotan.ens.fr> writes
>   I have to induce beta-galactosidase in a liquid culture of E.coli Y1089
>lysogen for a lambda gt11 phage containing a beta-galactosidase-fusion
>protein gene.
>   I have read in a protocole to use 10 mM IPTG, which seems to me an
>enormous amount, since I have to make a liter scale culture.
>   Is this concentration required? What is the minimal efficient
>concentration to fully induce beta-galactosidase synthesis?

We routinely use 0.5mM final concn. for pUC derivative expression
vectors but we have gone down to 0.1mM on some.

10mM is way too much.

My mind's made up. Don't confuse me with the facts!
Duncan Clark
DNAmp Ltd.

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