In article <genecutl-0810962054520001 at kosh-161b-2.berkeley.edu>,
genecutl at mendel.berkeley.edu (gc) wrote:
> I have a protein that I want to express which is toxic to E.coli. I
> would like to get my hands on an E. coli system in which cells
> are infected with a T7 polymerase-containing phage rather than adding
> IPTG to express an endogenous T7 polymerase. If anyone knows where I
> can find a system like this I would be most grateful.
>> ...doom is like the handle of a pot, | stop waiting, it's
> it's there, know it, have ice in your| already happened.
> tea, marry, have children, visit your| genecutl at mendel.berkeley.edu> dentist, do not scream at night even |
> if you feel like screaming... -cb |
If you have no luck in finding the phage you want (sorry, I don't have it
either) or you just want to give something a try, try adding some glucose
to your LB to keep the lacUV5 promoter repressed. We've had some luck
doing this and it allows us to keep the cultures around a bit longer.
University of California - Davis