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? Invitrogen pZErO-1 with Stratagen SURE cells

Steven Goldberg goldberg at bms.com
Wed Oct 9 11:17:16 EST 1996


In article <53dt0d$doq at mserv1.dl.ac.uk>, "Juergen Sasse (Path)"
<js1 at mole.bio.cam.ac.uk> wrote:

> I would like to use the pZErO-1 plasmid with SURE cells. Has anybody 
> tried it, and how did it work? Invitrogen say it might not work or not 
> very well as they have optimised it for their TOP10F' strain. I don't 
> want to use the TOP10F' cells because I am cloning untable DNA. Please 
> send an answer to the newsgroup as well as to myself. Thanx, Juergen.
> email: js1 at mole.bio.cam.ac.uk

I haven't tried using SURE cells with pZeroI, but since this strain
contains the lacIq repressor, it should be okay.

Steve



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