Dilution of Ascites for IF staining?

Barbara Baudoux Invite.histo at fundp.ac.be.
Wed Oct 9 10:34:42 EST 1996


Hello!
I'm a grad student and I want to use ascites to label CD antigens by
immunofluorescence on cultured cells. Could somebody suggest me a range of
dilutions of the liquid ascite before I start my labelling? I'll use a
FITC-conjugated secondary Ab to reveal binding.
Thanks a lot.

You can either send me an e-mail at <invite.histo at fundp.ac.be> or post in
this group.

Barbara.



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