IPTG concentration for induction?

Danièle EUSEBE deusebe at wotan.ens.fr
Wed Oct 9 09:56:13 EST 1996


   I have to induce beta-galactosidase in a liquid culture of E.coli Y1089
lysogen for a lambda gt11 phage containing a beta-galactosidase-fusion
protein gene.
   I have read in a protocole to use 10 mM IPTG, which seems to me an
enormous amount, since I have to make a liter scale culture.
   Is this concentration required? What is the minimal efficient
concentration to fully induce beta-galactosidase synthesis?

-- 
Daniele EUSEBE(Ecole Normale Supérieure-INSERM U293-Paris-France)
e-mail:deusebe at wotan.ens.fr




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