I haven't noticed much of a difference except that:
1. You only need half as much IpOH as EtOH in the ppt step.
2. IpOH ppt's in the extreme long run are cheaper (or so Qiagen says
so).
3. EtOH doesn't freeze as readily as an IpOH ppt if you're into
-20oC incubations, which I don't do anyway.
4. I've never seen a wash step that uses 70% IpOH.
Lou Cantolupo
GenVec, Inc.
Dept. Molecular Virology
Rockville, MD 20852
CANTOLUPO at GENVEC.COM
