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tissue stored at -20 C -- RNA still OK?

Juergen Brosius brosius at uni-muenster.de
Wed Oct 9 05:36:27 EST 1996

If we do not use fresh tissue for RNA preps we store it prior to prepping
at -70 C and never thaw it in between.  I have the option to use tissue of
interest that, however, had been stored at -20 C.  Does anyone have
experience concerning the state of the RNA extracted from such a source? 
I remember vaguely that a similar question was posted before but I did not
see the outcome.

Thanks in advance

                                          usual disclaimers
Juergen Brosius
Inst. f. eXp. Pathol.
University of Muenster
Von-Esmarch-Str. 56
D-48149 Muenster, Germany

Fax: +49  251  83 85 12
Tel: +59  251  83 85 11

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